Blood bank tests and methods
What tests are done when you donate blood
Mann—Whitney test was applied to compare the difference in strength of reactions of 55 incompatible crossmatches between the CTT and the CAT on the machine. This selection aimed at including the samples with antibodies so that the concordance of the two techniques and the strength of reaction could be compared for incompatible crossmatch results. In other studies validating automated systems, the most common identifiable cause of undetermined ABO and Rh typing was weak subgroups of ABO system. Methodology Blood grouping and crossmatching were first performed on Techno TwinStation machine. Statistical tests The percentage of concordant, discordant and uninterpretable results was calculated for grouping and crossmatching. However, sometimes, a patient may want to donate blood a couple of weeks before undergoing surgery, so that his or her blood is available in case of a blood transfusion. Abstract Context: The routine immunohematological tests can be performed by automated as well as manual techniques. At present, only testing for hepatitis C antibody is available. Mann—Whitney test was used to compare the strength of agglutination reaction by the two techniques. By performing pre-donation testing the infected prospective donor is spared the trouble of going through the donation process unnecessarily and the discard rate of donated blood is reduced. The mean incubation time is 6 to 8 weeks. It is estimated to be about 1 in , units of blood for HIV 4 , 1 in , units for hepatitis C virus and 1 in 63, units for hepatitis B virus.
The mean incubation time is 6 to 8 weeks. Aims: The present study aims to compare the results of manual and automated techniques for blood grouping and crossmatching so as to validate the automated system effectively.
To evaluate the time taken by each technique for blood grouping, 3 batches consisting of 1, 18 and 36 samples were run by both the techniques. This selection aimed at including the samples with antibodies so that the concordance of the two techniques and the strength of reaction could be compared for incompatible crossmatch results.
Blood bank tests and methods
There was discordance for 4 out of 0. Bacterial Contamination Bacterial contamination of blood can occur during collection. In case of a discrepancy between forward and reverse grouping, additional reagents anti-A1 and anti-H were used along with other guidelines for resolving the discrepancy. Shittu moc. The compared costs were also computed for both Pre-donation testing of all prospective donors and Post-donation testing of blood samples of donors who eventually donated blood. Platelet products carry the greatest risk 1 in units may have bacteria , because they are stored at room temperature. Department of Transfusion Medicine, Seth G. The donor units are then separated into various components and stored. Eight hundred of these prospective donors were deferred after simple rapid testing. Potential problems in selected patient populations can be prevented by transfusing CMV negative blood or frozen, deglycerolized RBC's.
Transfusing a contaminated unit may uncommonly result in severe sepsis 1 in, septic shock and death. After 10 min of centrifugation, the pictures were captured by the camera in the machine, and the interpretation was displayed.
This study was carried out to establish whether or not Pre-donation testing affords substantial cost savings without compromise to blood safety. The annual workload of the center includes more than 30, donor unit collection, and approximately 40, crossmatches.
Sample selection Blood grouping As a pilot study, a total of blood samples blood donors and recipient patients were selected for comparison of ABO and Rh blood grouping by the CTT and by the automated microplate LYRA system.
Conclusions: The automated system shows a high concordance of results with CTT and hence can be brought into routine use.
The study also aimed at identifying the areas responsible for maximum errors in automated techniques and to provide a possible solution to minimize the same. These were not interpreted by the LYRA system due to the lack of specialized anti-A1 and anti-H antisera gel cards as they are not a part of routine blood group testing.
These five samples were subjected to repeat the test on the automated system using the specialized anti-A1 and anti-H gel cards.
All processes and inputs were evaluated.
based on 59 review